Mueller Hinton Agar, together with the Mueller Hinton Broth (Cat. CL-1214), is used to test the antimicrobial susceptibility of rapidly growing aerobic organisms from clinical samples. It has become the standard medium for the Bauer Kirby method in accordance to the standards of the Clinical and Laboratory Standards Institute (CLSI) and European Committee and Antimicrobial Susceptibility Testing (EUCAST).

The main objective of in vitro antimicrobial susceptibility testing is to provide a guide for the therapeutic management of infectious diseases through the sensitivity or resistance of facultative aerobic and anaerobic pathogenic bacteria to different antimicrobial compounds. However, as it is impossible to predict the susceptibility of a bacterium responsible for a specific infection to antimicrobials, the antibiotic susceptibility tests carried out in the microbiological laboratory become an essential instrument for the therapeutic management of patients. In the medium, beef infusion and acid casein peptone to provide nitrogen, vitamins, minerals, and amino acids that are essential for growth. The starch absorbs any toxic metabolite produced by microbial growth and the bacteriological agar is the solidifying agent.