pOnebyOne FREE Bicistronic 20 rxn (1 µg)

pOnebyOne™ FREE Bicistronic Mammalian Expression Kit, extremely efficient lineal vector ready-to-clone your pcr gene and express your protein of interest

pOnebyOne™ are extremely efficient, accurate and flexible Bicistronic Mammalian Expression Kits that contains an expression cassette based in 2A sequence Breakthrough technology. pOnebyOne™ FREE allows co-expression of two genes of interest driven by human cytomegalovirus early promoter. It is based in 2A sequence from Thosea asigna Virus (TaV) which is flanked by two multiple cloning sites. T2A sequence leads to the highest level of protein expression at the second gene position compared to other 2As. The mechanism of 2A self-cleavage is a ribosome skipping process where the ribosome skips the formation of a glycil-prolyl peptide bond at the C-terminus of 2A. Stable mammalian cells could be selected by neomycinresistance. Both genes of interest must be in frame with T2A and between them. Successful skipping results in translation of two proteins: 2A upstream protein is attached to the 2A sequence (GSGEGRGSLLTCGDVEENPG) at C-terminus while 2A downstream protein is attached to one Pro at the N-terminus.

Main Features:

Complete solution: a directional cloning vector to clone and produce a protein of interest. Breakthrough technology: based in 2A sequence simultaneous expression of two proteins in mammalian cells (a protein of interest and reporter). Highly efficient: cloning system tested with up to 4 kb inserts. Time-saving cloning process: linearized vector ready-to-mix with your PCR fragment. Easy-to-use: facilitates the selection of positive cells expressing the recombinant gene of interest. Cost avoidance: avoids the use of restriction enzymes. Accurate: proven performance for most common cell lines. Flexible: allows transfection in difficult cell lines. Really low experimental background: lower than 1%. Convenient: available with different resistance marker cassettes. Directional cloning: of PCR with the gene of interest. Reporter checking: stoichiometric amount of your protein of interest and a reporter protein. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes: - 20 µL pOnebyOne™ FREE (50 ng/ µL) - as Closed Circular Vector

Applications: Protein Expression of intracellular, extracellular or transmembrane higher cells in an equimolecular to a surface marker that allows quantification.