Canvax DNase I EZ0018

DNase I, free of rnases.

DNase I is a recombinant endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release di-, tri- and oligonucleotide products (on average producing tetranucleotides) with 5’-phosphorylated and 3’-hydroxylated ends. It acts on single-stranded DNA, double-stranded DNA, RNA-DNA hybrids and chromatin. DNase I requires bivalent cations (Mg2? and Ca2?) for maximal activity.

Main Features:

Recombinant: bovine Pancreatic, purified from E. coli (29 kDa monomer). High specific activity: more than 2,000 Kunitz units per mg protein. High Quality: free of RNase and Proteinase contamination. Complete solution: supplied with 10x Reaction Buffer. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes : - 1 mL DNase I (10 mg/mL) - 1 mL Reaction Buffer (10x) * 10x Reaction Buffer: 100 mM Tris-HCl (pH 7.5 at 25°C), 25 mM MgCl2, 1 mM CaCl2

Applications: Removal of residual genomic DNA from RNA samples. Degradation of DNA template in transcription reactions. DNAse I footprinting. Perform Nick Translation.