Canvax pSpark® I DNA Cloning Kit C0001

pSpark® I DNA Cloning Kit, for highly efficient, accurate & robust general cloning from pcr high fidelity fragments, without toxic genes use

pSpark® I is a highly efficient, accurate and easy-to-use DNA cloning System based on a novel breakthrough technology to generate blunt vectors with a high blunt cloning efficiency. The vector is prepared by digestion of pSpark® at the EcoRV site before treating both ends to prevent vector self-ligation. The end treatment is supported by a proprietary know-how that guarantees a higher cloning efficiency than with simply a desphorylated vector.

Main Features:

Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions. Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding "suicide" strategies from toxic genes. Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation. High stability: eliminates cloning bias or pitfalls. Powerful: clone from less than 1 ng/kb, obtain 5-fold more positive colonies using 10x less DNA insert. Compatible with blue/white screening. Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers. Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert. Eliminates positive selection vector. High cost-saving: reduces your cloning costs as no expensive phosphorylated primers are needed. Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pSpark® I (20 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 200 µL T4 DNA Ligase Buffer (5x) - 150 µL PEG 6000 (10x) - 5 µL Insert Control 1 kb (20 ng/µL)

Applications: General cloning. Cloning of High Fidelity PCR amplified products. Production of ssDNA. Blue/white screening for recombinants. In vitro transcription from T7/SP6 dual-opposed promoters.