Canvax pSpark® IV DNA Cloning Kit C0004-S

pSpark® IV DNA Cloning Kit, for highly efficient, stable & powerful cloning under transcription-free conditions

pSpark® IV is a highly efficient, accurate and easy-to-use DNA cloning system that exploit its very low background feature for the expression of toxic genes under transcription-free conditions. In this vector, the lac promoter has been eliminated and therefore blue/white screening is not allowed (alpha-peptide coding region remains and you can find blue colony). The vector is ideal for cloning genes that produce toxic polypeptides by transcription/ translation.

Main Features:

Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions. Transcription-free. Easy-to-use: eliminate screening of recombinants due to its minumum background (lower than 1%). High stability: eliminates cloning bias or pitfalls. Time-saving protocol: avoids any step required after PCR, just 19 minutes from PCR to plating. Powerful: obtain 5-fold more positive colonies using 10-fold less DNA insert. Great versatility: compatible with any protocol, proofreading Polymerase, Competent Cells, ligation time or primers. Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert. Cost avoidance: removes expensive primer phosphorylation use. Eliminates positive selection vector. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pSpark® IV (20 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 200 µL T4 DNA Ligase Buffer (5x) - 150 µL PEG 6000 (10x) - 5 µL Insert Control 1 kb (20 ng/µL)

Applications: Cloning of high fidelity PCR amplified products. Production of ssDNA. In vitro transcription from T7/SP6 dual-opposed promoters. Cloning of toxic genes.