Canvax pSpark® V DNA Cloning Kit C0005

pSpark® V DNA Cloning Kit, for highly efficient, accurate & easy cloning with pbr322 and transcription-free conditions

pSpark® V is a highly efficient, accurate and easy-to-use DNA cloning system developed with low copy number, as a help for cloning of inserts with the highers kb. This low copy variant is also transcription-free, for the most demanding cloning tasks. In this vector, the lac promoter has been eliminated and therefore blue/white screening is not allowed (alpha-peptide coding region has been truncated).

Main Features:

Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions. Transcription-free. Easy-to-use: eliminate screening of recombinants due to its minumum background (lower than 1%). High stability: removes cloning bias or pitfalls. Time-saving protocol: avoids any step required after PCR, just 19 minutes from PCR to plating. Powerful: obtain 5-fold more positive colonies using 10-fold less DNA insert. Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers. Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert. Optimized: truncated alpha-peptide coding region. Cost avoidance: avoids expensive primer phosphorylation use. Eliminates positive selection vector. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pSpark® V (20 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 200 µL T4 DNA Ligase Buffer (5x) - 150 µL PEG 6000 (10x) - 5 µL Insert Control 1 kb (20 ng/µL)

Applications: Cloning of toxic genes. Cloning of unstable genes, for example genes with repeated sequences. Cloning of high fidelity PCR amplified products. Production of ssDNA. Blue/white screening for recombinants. In vitro transcription from T7/SP6 dual-opposed promoters.