Canvax pSpark® TA Done DNA Cloning Kit C0021-S

pSpark® TA Done DNA Cloning Kit, for efficient, stable & easy cloning of blended and non-proofreading pcr fragments with ecori and noti flanking the insertion site

pSpark® TA Done is efficient, stable and easy-to-use DNA cloning vector based on an improved TA technology that offers all of the advantages of pSpark® TA with the added convenience of recognition sites for EcoRI and NotI flanking the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion.

Main Features:

Convenient: recognition sites for EcoRI and NotI flanking the insertion site. Flexible: allows removing the desired insert DNA with other restriction digestion. Efficient: more than 600 white positive colonies expected under optimal conditions. Stable: without cloning bias due to transcription of toxic genes. Easy-to-use: eliminates screening of recombinants due to its low background (4%). Fast protocol: ligation time from 60 minutes to overnight. Compatible: with direct cloning of PCR products. Great versatility: compatible with any competent cell, primer design or particular proofreading DNA Polymerase. Cost avoidance: avoids primer phosphorylation. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pSpark® TA Done (50 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 200 µL T4 DNA Ligase Buffer (5x) - 5 µL Insert Control 1 kb (20 ng/µL)

Applications: Cloning of non-proofreading PCR fragments. Production of ssDNA. Blue/white screening for recombinants. In vitro transcription from T7/SP6 dual-opposed promoters.