Canvax pColiExpress™ IV Cloning & Expression Kit BE017

pColiExpress™ IV Cloning & Expression Kit, for a highly efficient, fast & versatile protein expression (t4 dna ligase based) in e. coli from pcr fragment cloned

pColiExpress™ IV Cloning &amp, Expression Kit is a DNA Cloning System for Protein Expression in E.coli. pColiExpress™ technology allows efficiently cloning DNA fragments and the quick production of a large quantity of any desired Protein. pColiExpress™ IV contains all elements required for expression of large quantity of any desired protein by T7 RNA Polymerase Inducible System and a His6 tag at COOH end that allow the protein purification. It contains at NH2 end cmyc tag followed by PreScission Protease cleavage site, too. The vector has also an f1 origin of replication, an ampicillin resistance cassette, and a pBR322 origin of replication.

Main Features:

Ready-to-use vectors. Highly efficient cloning system. Special design that allows the cell to house larger numbers of copies than other plasmids with ori pBR322. Linearized vector: ready for ligation with your PCR amplified with the recommended primers. Minimum background: lower than 1%. Time-saving protocol: avoids any step required after PCR. Higher protein expression than other suppliers. Cost avoidance: avoids the use of expensive phosphorylated primers. Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pColiExpress™ linearized vector (50 ng/µL) - 200 µL T4 DNA Ligase Buffer (5x) - 20 µL T4 DNA Ligase (5 U/µL) - 10 µL Control Insert DNA (30 ng/µL) - 5 µL pColiExpress™ Control (50 ng/µL) Plus Version Includes for 20 rxn - 20 µL pColiExpress™ linearized vector (50 ng/µL) - 200 µL T4 DNA Ligase Buffer (5x) - 20 µL T4 DNA Ligase (5 U/µL) - 10 µL Control Insert DNA (30 ng/µL) - 5 µL pColiExpress™ Control (50 ng/µL) - 20 µL EcoRV (10 U/µL) - 20 µL Hind III (10 U/µL) - 1.5 mL Buffer C (10x)

Applications: Cloning of PCR fragments for subsequent expression of proteins in E. coli. Expression of Proteins under the control of the T7 promoter. Protein expression in BL21 (DE3) or BL21 (DE3) (pLys).