Canvax Annexin V Apoptosis Detection Kit CA012

Annexin V Apoptosis Detection Kit, for a convenient, easy & fast method for apoptosis detection by flow cytometry and fluorescence microscopy

Annexin V Apoptosis Detection Kits are a convenient, easy-to-use and safe method for Apoptosis Detection. Annexins are a family of calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS). Externalization of phosphatidylserine residues on the outer plasma membrane of apoptotic cells allows detection via Annexin V. Once the apoptotic cells are bound with labelled Annexin V, it can be visualized with fluorescent microscopy or cytometry. Since loss of membrane integrity is a pathognomonic feature of necrotic cell death, necrotic cells will stain with specific membrane-impermeant nucleic acid dyes such as propidium iodide, the membrane integrity of apoptotic cells can be demonstrated by the exclusion of these dyes.

Main Features:

Easy and one step protocol: get results in 9 minutes. Detection Method: Flow cytometry (Ex = 488 nm

Includes: Includes for 100 assays: -500 µL Labeled Annexin V -50 mL Binding Buffer (10x) -1 mL Propidium Iodide

Applications: Detect early/middle stages of Apoptosis. Differentiate apoptosis from necrosis.