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Home > Laboratory > Life Sciences > Molecular Cell Biology > Cell Based Assays > Reporter Gene Assays > Canvax ONPG - Galactosidase Assay Kit CA080

Canvax ONPG - Galactosidase Assay Kit CA080
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Canvax ONPG - Galactosidase Assay Kit CA080

PART NO: CX-CA080

ONPG - Galactosidase Assay Kit, optimized, stable & convenient tool to quickly measure the levels of active ß-galactosidase

ONPG B-Galactosidase Assay Kit is an optimized, stable and convenient tool to quickly measure the levels of active ß-galactosidase expressed in cells transfected with plasmids expressing Lac Z. Lac Z is often used reporter gene in experiments transfection because the ß-galactosidase is very resistant to proteolytic degradation and its activity is easily measured. ß-galactosidase performs the hydrolysis of orthonitrophenyl-ß-D-galactopyranoside (ONPG) to the ortho-nitrophenol (ONP). This ONP produces a bright yellow colour that is detected at an absorbance of 420 nm. The concentration of ß-galactosidase is proportional to colour produced.

Main Features:

Proven performance to quantify high expression level of beta-Gal. Very stable: resistant to proteolytic degradation and easily assayed. Convenient for all transfection assays. Versatile: proven performance for cultured cells and tissues. Fast and easy protocol. Cost-effective. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 500 assays: - ONGP Substrate solution - DTT - Buffer Lysis - Buffer Assay - Buffer Stop - ß-galactosidase enzyme

Applications: Measurement of ß-Galactosidase activity in the lysates of transfected cells.


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