Canvax T4 DNA Polymerase P0042

T4 DNA Polymerase, recombinant, from e.coli cells with a cloned gene 43 of bacteriophage t4 (5 u/µl)

Bacteriophage T4 DNA Polymerase is a DNA-directed 5’ to 3’ DNA polymerase. It is the product of gene 43 from bacteriophage T4 and is therefore often referred to as T4 gp43 DNA Polymerase. The enzyme catalyzes the polymerization of deoxynucleotide triphosphates in a 5’ to 3’ direction. It possesses very active 3’ to 5’ exonuclease activity that is more active on single than double stranded DNA, T4 DNA polymerase has no 5’ to 3’ exonuclease activity. For polymerase activity the enzyme requires DNA with a 5’ protruding end and a high concentration of TruePure™ dNTPs.

Main Features:

Recombinant: isolated from a recombinant source (E.coli cells with a cloned gene 43 of bacteriophage T4). Powerful: stronger 3'-5' exonuclease activity on single-stranded than on double-stranded DNA. Complete solution: supplied with 10x Reaction Buffer. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 100 U: - 20 µL T4 DNA Polymerase (5 U/µL) - 1 mL Reaction Buffer (5x)

Applications: Generation of blunt double-stranded DNA from DNA containing 5' overhangs. Generation of blunt double-stranded DNA from DNA containing 3' overhangs. 5'-end or 3'-end labeling of double-stranded DNA. In vitro mutagenesis.