Canvax pSpark® III DNA Cloning Kit C0003-S

pSpark® III DNA Cloning Kit, for highly efficient, accurate & easy cloning with ampicillin and kanamycin resistance

pSpark® III is a highly efficient, accurate and easy-to-use DNA cloning system that combines Ampicillin and Kanamycin resistance. Ideal for cloning PCR products amplified from any plasmid vector without the need to gel-purify bands to eliminate the background due to the template vector used for PCR.

Main Features:

Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions. Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding "suicide" strategies from toxic genes. Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation. High stability: eliminates cloning bias or pitfalls. Powerful: clone from less than 1 ng/kb, obtain 5-fold more positive colonies using 10x less DNA insert. Compatible with blue/white screening. Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers. Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert. Eliminates positive selection vector. High cost-saving: reduces your cloning costs as no expensive phosphorylated primers are needed. Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pSpark® III (20 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 200 µL T4 DNA Ligase Buffer (5x) - 150 µL PEG 6000 (10x) - 5 µL Insert Control 1 kb (20 ng/µL)

Applications: Cloning directly from PCR using plasmid cloned genes as template. Unpurified PCR cloning. Cloning of high fidelity PCR amplified products. Production of ssDNA. Blue/white screening for recombinants. In vitro transcription from T7/SP6 dual-opposed promoters.