Canvax pSpark® Done DNA Cloning Kit C0006-S

pSpark® Done DNA Cloning Kit, for highly efficient, accurate & easy cloning of pcr fragments with ecori and noti flanking the insertion site

pSpark® Done is a highly efficient, accurate and easy-to-use DNA cloning system designed for cloning of blunt ended DNA with very high efficiency. The MCS of the pSpark® Done vector incorporates sequences on either side of the insert that are recognized by the restriction enzymes NotI and EcoRI. This allows the insert DNA to be removed with a single restriction digest using either of these enzymes

Main Features:

Optimized: recognition sites for Not I and EcoR I at either side for the insertion of the cloning point. Flexible: allows removing the desired insert DNA with other restriction digestion. Unprecedented efficiency: more than 2,500 positive colonies expected under optimal conditions. Easy-to-use: eliminate screening of recombinants due to its its minumum background (lower than 1%). Time-saving protocol: avoids any step required after PCR, just 19 minutes from PCR to plating. Powerful: obtain 5-fold more positive colonies using 10-fold less DNA insert. High stability: eliminates cloning bias or pitfalls. Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers. Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert. Eliminates positive selection vector. Cost avoidance: removes expensive primer phosphorylation use. Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pSpark® Done (20 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 200 µL T4 DNA Ligase Buffer (5x) - 150 µL PEG 6000 10x - 5 µL Insert Control 1 kb (20 ng/µL)

Applications: Cloning of High Fidelity PCR amplified products. Production of ssDNA. Blue/white screening for recombinants. In vitro transcription from T7/SP6 dual-opposed promoters. One restriction enzyme allows gene fragment excision.