Canvax pSpark® Universal DNA Cloning Kit C0019

pSpark® Universal DNA Cloning Kit, highly efficient, sccurate & easy-to-use system compatible with blunt and ta dna cloning

pSpark® Universal is a highly efficient, accurate and easy-to-use DNA Cloning kit ideal for a broad range of PCR fragments cloning applications. There is a range of DNA Polymerases available that do not generate PCR products with identical ends: proofreading DNA polymerases leave blunt ends while blends of polymerases and non-proofreading DNA polymerases leaves 3´A overhangs. Therefore, it is necessary to employ different vectors to clone both kinds of PCR fragments. pSpark® Universal DNA cloning kit has been designed to save time, looking for a kit for several cloning scenarios. It is mainly composed of two cloning vectors which allow blunt or TA DNA cloning. For blunt DNA cloning and TA DNA cloning, pSpark® II DNA Cloning vector and pSpark® TA DNA Cloning vector, respectively, are included.

Main Features:

Compatible with Blunt and TA DNA cloning: it is composed by pSpark® II and pSpark® TA DNA Cloning vectors. Convenient: ideal for a broad range of PCR fragments cloning applications. Versatile: compatible with any DNA Polymerase. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pSpark® II (20 ng/µL) - 20 µL pSpark® TA (50 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 200 µL T4 DNA Ligase Buffer (5x) - 150 µL PEG 6000 (10x) - 1 kb Insert Control (20 ng/µL) - Insert Control TA (30 ng/µL)

Applications: Cloning of high fidelity PCR amplified products into pSpark® II Blunt DNA cloning vector. Cloning of non-proofreading PCR fragments into pSpark® TA DNA Cloning vector. Production of ssDNA. In vitro transcription from T7/SP6 dual-opposed promoters.