Canvax pSpark® TA DNA Cloning Kit C0020-S

pSpark® TA DNA Cloning Kit, for efficient, stable & easy cloning of non-proofreading pcr fragments or pcr from a blend of enzymes

pSpark® TA is efficient, stable and easy-to-use DNA cloning vector based on an optimized TA technology for cloning single 3 ´-adenine overhanging DNA. The vectors are prepared by digestion of pSpark® TA at EcoRV site and the subsequent addition of a single thymidine at each 3 ´- end to allow cloning Taq DNA Polymerase amplified DNA fragments. Its exclusive procedure offers greater efficiency and less background of blue colonies than the others TA vectors.

Main Features:

Efficient: more than 600 white positive colonies expected under optimal conditions. Easy-to-use: eliminates screening of recombinants due to its low background (4%). High stability: vector without cloning bias due to transcription of toxic genes. Fast protocol: ligation time from 60 minutes to overnight. Compatible: with direct cloning of PCR products. Great versatility: compatible with any competent cell, primer design or particular proofreading DNA Polymerase. Cost avoidance: avoids primer phosphorylation. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pSpark® TA (50 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 200 µL T4 DNA Ligase Buffer (5x) - 5 µL Insert Control 600 bp (30 ng/µL)

Applications: Cloning of non-proofreading PCR fragments. Production of ssDNA. Blue/white screening for recombinants. In vitro transcription from T7/SP6 dual-opposed promoters.