Rogosa SL Agar is used for the isolation, enumeration, and identification of lactobacilli in oral bacteriology, faeces, vaginal specimens, and foodstuffs. This selective medium has been modified by Rogosa to contain high levels of sodium acetate and ammonium citrate at a low pH. This inhibits most microorganisms, including streptococci and moulds and limits swarming but allows the growth of lactobacilli. Sucrose, arabinose, and dextrose are fermentable carbohydrates as carbon and energy sources. Tryptone provides nitrogen, vitamins, minerals, and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. Sulphate salts provide inorganic ions. Polysorbate 80 is a surfactant and is incorporated to neutralize phenols, hexachlorophene, and formalin. Monopotassium phosphate acts as a buffer system. Bacteriological agar is the solidifying agent.
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