Pplo Agar Base W/O Crystal Violet 1140 Condalab

PPLO (Pleuro Pneumonia-Like Organisms) Agar Base w/o Crystal Violet is used in the study of the growth requirements of Mycoplasma. It can also be used for the identification and cultivation of this organism. Peptone and beef heart infusion provide the nutrients for growth: nitrogen, vitamins, minerals, and amino acids, whilst the sodium chloride provides the osmotic balance. Crystal violet is not included in this formula since it is inhibitory on some Mycoplasma. Bacteriological agar is the solidifying agent. PPLO colonies have a round shape and a dense centre with a less dense periphery, giving a “fried egg” appearance on agar.