Chromogenic Cronobacter Isolation Agar Condalab 1446

Chromogenic Cronobacter Isolation Agar is used for the detection of Cronobacter spp. in food and ingredients that are intended for human consumption, animal feeding, and environmental samples. It is ISO 22964 compliant which descrives a horizontal method for the detection of Cronobar spp. and recommends that this medium be used in the isolation of Cronobacter spp.

In the medium, tryptone provides nitrogen, vitamins, minerals, and amino acids essential for growth and yeast extract is also a source of vitamins, particularly the B-group, essential for bacterial growth. The sodium chloride provides essential electrolytes for transport and osmotic balance and the sodium deoxycholate inhibits the gram-positive flora. Sodium thiosulfate increase the selectivity and the recovery of Cronobacter and Enterobacter species. 5-Bromo-4-chloro-3-indolyl a-D-glucopyranoside is the chromogenic substrate.

Cronobacter is considered to be an emerging pathogen responsible for severe meningitis and necrotic enterocolitis in un-weaned babies that can be the cause of mortality rate between 40-80%. The pathogenicity of Cronobacter for un-weaned babies’ makes it vital to review the manufacturing process of the milk-based products for babies, guaranteeing the absence of the bacteria in the final product. It is also important that prevention measures are put in place in hospital to include the sanitary hygiene of prepared food, reducing preparation and administration so to stop the spread of microorganisms.