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Home > Life Science > Cell Culture > Classical Media > Dulbeccos MEM Low Glucose Media > DMEM Low Glucose without L-Glutamine Bioconcept 1-25F01-I

DMEM Low Glucose without L-Glutamine Bioconcept 1-25F01-I
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DMEM Low Glucose without L-Glutamine Bioconcept 1-25F01-I

PART NO: BC-1-25F01-I
Price: €13.44
Out of Stock (Ships in approx. 1 week)

Dulbecco's MEM is the most widely used modification of BME. It contains four times the concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added and the bicarbonate concentration was increased. The standard formula is with 1000 mg/L glucose, the “high glucose” variation with 4500 mg/L. Dulbecco’s MEM was originally developed for the culture of mouse embryonic cells. Today it finds a broad application for serum free culture of normal and transformed mouse and chicken cells, e.g. 1:1 with Ham's F-12.


Inorganic Salts mg / L
CaCl2 2H2O 264
Fe(NO3)3 9H2O 0.1
MgSO4 7H2O 200
KCl  400
NaCl 6400
NaH2PO4 H2O  125
NaHCO3 3 700
Other Components  
D(+)-Glucose 1000
Phenol Red 15
Pyruvic acid sodium salt 110
Amino Acids  
Glycine 30
L-Arginine HCl 84
L-Cystine 48
L-Histidine HCl H2O 42
L-Isoleucine 105
L-Leucine 105
L-Lysine HCl 146
Amino Acids mg / L  
L-Methionine 30
L-Phenylalanine 66
L-Serine 42
L-Threonine 95
L-Tryptophan 16
L-Tyrosine 72
L-Valine 94
Vitamins  
Choline chloride 4
D-Pantothenic acid calcium salt 4
Folic acid  4
myo-Inositol 7.2
Nicotinamide 4
Vitamin B1 HCl 4
Vitamin B2 0.4
Vitamin B6 HCl 4
To be added separately  
L-Glutamine 584

 

ManufacturerBioconcept
Manufacturer P/n1-25F01-I
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