Tsn Agar 1075 Condalab

Tryptone Sulphite Neomycin Agar is a selective medium that can be used in tubes or plates for the identification and enumeration of Clostridium perfringens in foods and other materials, especially from mixed contaminating flora. The Casein peptone provides nitrogen, vitamins, minerals, and amino acids essential for growth. Yeast extract is source of vitamins, particularly the B-group. Ferric citrate and Sodium sulphite are H2S indicators. C. perfringens reduces the sulphite to sulphide which reacts with the iron and forms a black iron sulphide precipitate, seen as black colonies. Bacteriological agar is the solidifying agent. Polymyxin and Neomycin sulphates inhibit the growth of the majority of Enterobacteria and Clostridium bifermentans. The incubation at a temperature of 46°C allows specific and quantitative results.