Fluorogenic EC Agar with MUG 500g Condalab 1180

EC with MUG Fluorogenic Agar has a similar formula to EC Medium but an additional 4 methylumbelliferyl- ß-D-glucuronide (MUG) that is recommended for the membrane-filter technique for the detection of E. coli. Faecal contamination is a serious issue as it can cause contracting diseases from pathogens. The medium improves detection methods of coliform groups, in particular E. coli and can be used in investigating drinking water, wastewater, and general water quality monitoring to help prevent the spread of disease. 

The medium can be used at 35±2 ºC for detection of coliform organisms or at 44.5 ºC for isolation of E. coli. The Bile salts act as selective agent inhibiting Gram-positive bacteria, bacilli and enterococci but allowing E. coli to develop. The Potassium salts have a high buffering capacity and tryptose provides the nutrients for growth and Lactose is the fermentable carbohydrate as carbon and energy source. Sodium chloride maintains the osmotic balance abnd the bacteriological agar is the solidifying agent. E. coli produces enzyme ß-D-glucuronidase that hydrolyzes MUG to yield a fluorogenic product that is detectable under long-wave (366 nm) UV light.