Urea Indol Broth ISO 10273 Condalab 1227

Urea Indole Broth is for the differentiation of Enterobacteria based on urease and indole production and the transdeamination of tryptophan (TDA). This medium can be used for the determination of urease and indole production by Enterobacteriaceae as well as microorganisms of the families of Brucella, Bacillus, Micrococcus, Mycobacteria and Proteus.

L-Tryptophan is an essential amino acid and is converted to skatole and indole. Sodium chloride maintains the osmotic balance and potassium phosphates act as a buffer system. Urea is a source of nitrogen for those organisms producing urease. Phenol red is the pH indicator. This medium is recommended for the presumptive identification of Yersinia enterocolitica.